Virus-induced gene silencing (VIGS) is a powerful method to study gene function in plants. Tobacco rattle virus (TRV)-based VIGS vector is the most efficient VIGS vector so far. This method was originally developed by the Dinesh-Kumar's group (Liu et al. https://www.diegojavierfares.com/amazing-price-Kicker-48CDF104-Comp-10-Down-Firing-Loaded-Subwoofer-Enclosure-special-find/