All outcomes had been derived from your linear amplification curve and normalized to β-actin. The ΔΔCT method was used to calculate the fold improve in αseven-nAChR expression. Moreover, we selected to irradiate cells 24 h right after mobile seeding and drug administration to make sure complete cell attachment right before irradiation. https://garrettxchmo.total-blog.com/getting-my-rat-to-work-51162976